ABSTRACT
Recombinant PRRSV △2ORF5 gene was constructed using DNA shuffling from four genetically different strains of PRRSV to study its heterologous cross-neutralizing ability. The △2ORF5 mutant gene was cloned into the vector pET-32a and transferred into E. coli BL21. SDS-PAGE confirmed that the molecular weight of the recombinant △2ORF5 was about 42 kDa, consistent with the predicted result. Then the purified recombinant protein was injected into BALB/c mouse to obtain polyclonal antibody. Western blotting analysis with mouse-anti-△2ORF5 polyclonal serum indicated that the parental virus recombinant GP5 protein reacted with the specific antibodies. Four parental viruses could be inhibited by the anti-△2ORF5 polyclonal antibody and the inhibition rates were higher than 53%. This work has laid a foundation for further development vaccine for PRRSV.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the Zedoary oil on A549 cell line of collagen deposition cat D and cat K expression.</p><p><b>METHOD</b>The A549 cell line were treat by Zedoary oil on four different concentrations (0, 40, 80, 120 mg x L(-1)) in different time. Dynamic changes of collagen in A549 cell using Picric-sirius red method. Cat D and Cat K expression of level were detected by using western blot.</p><p><b>RESULT</b>The collagen content showed that Zedoary oil had an inhibitory effect on the deposition of A549 cells. The results of western blot showed that the expression of cat D and cat K were up-regulated significangly in A549 cells of Zedoary oil groups compared with that in controls.</p><p><b>CONCLUSION</b>A549 cell of collagen deposition were reduced by Zedoary oil. The effects may due to the up-regulation of cat D and cat K.</p>
Subject(s)
Animals , Blotting, Western , Cathepsin D , Metabolism , Cathepsin K , Metabolism , Cell Line, Tumor , Collagen , Metabolism , Curcuma , Chemistry , Gene Expression Regulation, Neoplastic , Plant Oils , Pharmacology , Up-RegulationABSTRACT
OBJECTIVE To investigate the drug-resistance rate of strains isolated from nosocomial infection patients with Acinetobacter spp.METHODS A total of 198 patients with Acinetobacter spp nosocomial infection were studied retrospectively from 2003 to 2005.The antibiotic resistance profiles were specifically analyzed.RESULTS The strains of Acinetobacter spp were mainly isolated from sputum(74.75%) and wound secretion(11.61%) specimens.There were no significant differences in the resistance of Acinetobacter spp against antibiotics in 3 years except piperacillin/tazobactam and meropenem.The resistance rate of all 198 strains to 13 kinds of antibiotics were as following:imipenem/cilastatin 2.53%,meropenem 2.02%,cefoperazone/sulbactam 30.81%,piperacillin/tazobactam 38.38%,amikacin 53.54%,cefepime 61.11%,levofloxacin 66.67%,ceftazidime 66.16%,ciprofloxacin 68.69%,gentamicin 67.68%,sulfamethoxazole/trimethoprim 63.64%,piperacillin 70.72%,and aztreonam 85.35%.CONCLUSIONS It is indicated that Acinetobacter spp are highly resistant to the common antibiotics,but still sensitive to imipenem/cilastatin and meropenem.